SP6 RNA Polymerase from Promega

Product Specs
ItemSP6 RNA Polymerase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP1081
Concentration10–20u/µl
Quantity5,000u

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100µl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl2, 10mM DTT, 10mM NaCl, 2mM spermidine, 0.05% Tween®-20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5µCi [3H]CTP, and 2µg supercoiled pGEM®-5Zf(+) Vector DNA.

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Mechanically sensitive HSF1 is a key regulator of left-right symmetry breaking in zebrafish embryosiScienceOctober 20, 2023Jing Du, Shu-Kai Li, Liu-Yuan Guan, Zheng Guo, Jiang-Fan Yin, Li Gao, Toru Kawanishi, Atsuko Shimada, Qiu-Ping Zhang, Li-Sha Zheng, et al.Science,HXP1868 Q5 High-fidelity PCR kit,NEB,NEB T7 RNA Polymerase,Promega,P2075 SP6 RNA Polymerase,Promega,P1085 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, MDCK,ATCC,NBL-2 ,, Experimental models

(2) RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancerInternational Journal of OncologyAugust 11, 2023Jia Yuan, Wencai Guan, Xin Li, Fanchen Wang, Huiqiang Liu, Guoxiong Xusite (TSS)] were amplified and ligated into the pGL4-Basic vector (Promega Corporation) to generate P1081 and P780 plasmids. 293T cells were cotransfected with pGL4 and the control Renilla luciferase vector

(3) Wnt/β-Catenin Signaling Pathway Is Necessary for the Specification but Not the Maintenance of the Mouse Retinal Pigment EpitheliumMolecules and CellsJuly 31, 2023Jong-Myeong Kim, Kwang Wook Min, You-Joung Kim, Ron Smits, Konrad Basler, Jin Woo Kimprobes were prepared from pGEM-Axin2 plasmid DNA by in vitro transcription using SP6 RNA polymerase (P1085; Promega, USA). The probes were detected with an alkaline phosphatase-conjugated anti-DIG antibody (11093274910,

(4) WDR5 is required for DUX4 expression and its pathological effects in FSHD muscular dystrophyNucleic Acids ResearchJune 9, 2023Emanuele Mocciaro, Roberto Giambruno, Stefano Micheloni, Filippo M Cernilogar, Annapaola Andolfo, Cristina Consonni, Maria Pannese, Giulia Ferri, Valeria Runfola, Gunnar Schotta, et al.antisense RNA, the Sp6 polymerase was instead used, according to the manufacturer instruction (Promega, # P1085). Transcripts were treated with RNase-free DNase (Promega, #M6101) for 1 h at 37°C, purified with TRIzol

(5) Dopey2 and Pcdh7 orchestrate the development of embryonic neural stem cells/ progenitors in zebrafishiScienceMarch 17, 2023Xiao Y, Hu M, Lin Q, Zhang T, Li S, Shu L, Song X, Xu X, Meng W, Li X, et al.Kit,Beyotime,P2078 Universal DNA Purification Kit,TIANGEN,4992197 T7 RNA Polymerase,Promega,P2075 SP6 RNA Polymerase,Promega,P1085 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(6) A calcitonin receptor-expressing subregion of the medial preoptic area is involved in alloparental tolerance in common marmosetsCommunications BiologyNovember 21, 2022Kazutaka Shinozuka, Saori Yano-Nashimoto, Chihiro Yoshihara, Kenichi Tokita, Takuma Kurachi, Ryosuke Matsui, Dai Watanabe, Ken-ichi Inoue, Masahiko Takada, Keiko Moriya-Ito, et al.5′ end (5′-ATTTAGGTGACACTATAGAA -3′). The antisense probes were transcribed by SP6 RNA polymerase (P1085; Promega) in the presence of digoxigenin-labelled UTP (Dig labelling mix; Roche Diagnostics, Basel,

(7) Developmental and evolutionary comparative analysis of a regulatory landscape in mouse and chickenDevelopmentJune 15, 2022Aurélie Hintermann, Isabel Guerreiro, Lucille Lopez-Delisle, Christopher Chase Bolt, Sandra Gitto, Denis Duboule, Leonardo Beccarirestriction enzymes and transcribed in vitro with T3 (Promega, P2083), T7 (Promega, P2078) or Sp6 (Promega, P1085) RNA polymerase (Table S3) and the Dig-RNA labelling mix (Roche). The probes were purified using the

(8) Remnants of the Balbiani body are required for formation of RNA transport granules in Xenopus oocytesiScienceMarch 18, 2022Yang C, Dominique GM, Champion MM, Huber PW.A,Sigma Aldrich,A8674 RNasin,Promega,N2511 T7 RNA polymerase,Promega,P2075 SP6 RNA polymerase,Promega,P1085 Ribo m7G Cap Analog,Promega,P1711 Oligomycin A,Sigma Aldrich,75351 Nocodazole,Sigma Aldrich,M1404 Leibovitz

(9) Rhein Protects Against Severe Acute Pancreatitis In vitro and In vivo by Regulating the JAK2/STAT3 PathwayFrontiers in PharmacologyMarch 17, 2022Xiaofang Yang, Huan Geng, Lijiao You, Lin Yuan, Jialei Meng, Yuhui Ma, Xuelian Gu, Ming LeiKits (Cat. No. A5001) were purchased from Promega (United States). Phosphatase inhibitors (Cat. No. P1081), SDS–PAGE gel preparation kits (Cat. No. P0012A) and ECL hypersensitive colour development kits (Cat.

(10) The ribosome inhibitor chloramphenicol induces motility deficits in human spermatozoa: A proteomic approach identifies potentially involved proteinsFrontiers in cell and developmental biologyJanuary 1, 2022Bisconti M, Leroy B, Gallagher MT, Senet C, Martinet B, Arcolia V, Wattiez R, Kirkman-Brown JC, Simon JF, Hennebert E.used to produce single stranded digoxigenin (DIG)-labelled RNA probes with the T7 (P2075) and Sp6 (P1085) transcription polymerases from Promega. Transcription was performed after manufacturer’s instructions

(11) Transcriptional repression by FEZF2 restricts alternative identities of cortical projection neuronsCell reportsJune 22, 2021Tsyporin J, Tastad D, Ma X, Nehme A, Finn T, Huebner L, Liu G, Gallardo D, Makhamreh A, Roberts JM, et al.Monohydrate,Sigma-Aldrich,C0706 Horse Serum,GIBCO,16050-114 T7 Polymerase,New England Biotechnology,M0251 SP6 Polymerase,Promega,P1085 DIG-Labeled Nucleotides,Sigma-Aldrich,11277073910 NBT/BCIP stock solution,Sigma-Aldrich,11681451001 SpeI

(12) Modified in situ Hybridization Chain Reaction Using Short Hairpin DNAsFrontiers in Molecular NeuroscienceMay 12, 2020Yousuke Tsuneoka, Hiromasa Funato(5′-ATTTAGGTGACACTATAG-3′) and (5′-TAATACGACTCACTATAGGG-3′). The probe was transcribed by SP6 RNA polymerase (P1085; Promega) in the presence of digoxigenin-labeled UTP (Dig labeling mix; Roche Diagnostics, Switzerland),

(13) Exercise enhances motor skill learning by neurotransmitter switching in the adult midbrainNature communicationsMay 4, 2020Li HQ, Spitzer NC.complementary RNA (cRNA) probes were synthesized with T7 (Promega, P2075) or Sp6 polymerases (Promega, P1085) and labeled with digoxigenin (Roche, 11175025910). Hybridization was performed with 1 to 5 μg/ml cRNA

(14) Long noncoding RNA PAHAL modulates locust behavioural plasticity through the feedback regulation of dopamine biosynthesisPLoS geneticsApril 1, 2020Zhang X, Xu Y, Chen B, Kang L.and PAH (biotin–PAH) were synthesised by using a T7/SP6 RNA polymerase kit (Promega, WI, USA, P2075, P1085) and DIG RNA labelling mixture/biotin RNA labelling mix (Roche, Mannheim, Germany, 11277073910, 11685597910).

(15) Dose-Dependent AGO1-Mediated Inhibition of the miRNA165/166 Pathway Modulates Stem Cell Maintenance in Arabidopsis Shoot Apical MeristemPlant CommunicationsJanuary 1, 2020Fei Du, Wen Gong, Sonia Boscá, Matthew Tucker, Hervé Vaucheret, Thomas Lauxcatalog no. 11277073910). Antisense and sense probes were synthesized using SP6 (Promega, catalog no. P1085) or T7 RNA polymerase (Promega, catalog no. P2075). The long probes were then hydrolyzed to an average

(16) A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization ProtocolBIO-PROTOCOLSeptember 5, 2019Chiara Sinigaglia(For probe synthesis) 15. T7/SP6/T3 RNA polymerase (Promega, catalogue number, respectively: P2075/ P1085/ P2083), storage: -20 °C (For probe synthesis) 16. RQ1 RNase-free DNase (Promega, catalog number: M6101),

(17) Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNAProceedings of the National Academy of SciencesAugust 13, 2019Nadiya Khyzha, Melvin Khor, Peter V. DiStefano, Liangxi Wang, Ljubica Matic, Ulf Hedin, Michael D. Wilson, Lars Maegdefessel, Jason E. FishEasy plasmid (SI Appendix, Table S2). In vitro transcription was performed using SP6 RNA Polymerase (P1085, Promega) and T7 RNA Polymerase (P2075, Promega) with the Biotin RNA Labeling Mix (Roche) using 1 µg

(18) An Isoprene Lipid-Binding Protein Promotes Eukaryotic Coenzyme Q BiosynthesisMolecular CellFebruary 1, 2019Danielle C. Lohman et al.Performance Resin,GE,28935599 Sypro Orange (5000X),Thermo Fisher,S-6650 SP6 RNA polymerase,Promega,P1085 RNasIn,Promega,N2111 Creatine kinase,Roche,10736988001 Geranylgeraniol,Sigma,G3278 Critical Commercial

(19) Multisensory Logic of Infant-Directed Aggression by MalesCellDecember 13, 2018Yoh Isogai, Zheng Wu, Michael I. Love, Michael Ho-Young Ahn, Dhananjay Bambah-Mukku, Vivian Hua, Karolina Farrell, Catherine Dulac,New England Biolabs or Promega,M0251S/L or P2075 Sp6 RNA polymerase,New England Biolabs or Promega,M0207S/L or P1085 T3 RNA polymerase,Promega,P2083 NBT/BCIP,Promega,S3771 Dig RNA labeling mix,Roche,11277073910 Fluorescein RNA labeling mix

(20) Pof8 is a La-related protein and a constitutive component of telomerase in fission yeastNature CommunicationsDecember 1, 2018Diego J. Páez-Moscoso, Lili Pan, Rutendo F. Sigauke, Morgan R. Schroeder, Wen Tang, Peter BaumannCi mmol−1), 1 μg DNA template, 40 U RNasin (Promega, N2111), and 1.9 U SP6 RNA polymerase (Promega, P1085) in a 10 μl volume. Reactions were incubated at 37 °C for 3 h (time optimized to maximize the amount

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